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Contracted analysis service for gene expression "NET-CAGE"

The NET-CAGE analysis that inputs the "NET-RNA" currently being synthesized. New service starting in the fall of 2019! An evolved version of CAGE analysis.

In the conventional "CAGE" analysis, total RNA is input, allowing for the following: - RNA expression analysis (serving the same role as general RNA-seq) - Identification of transcription start sites (the greatest strength of CAGE, which is insufficient with RNA-seq) - Searching for transcription factor binding motifs based on the identified transcription start site data (unique to CAGE, a reverse search function of ChIP-seq) The central principle of library preparation is the "cap trap method," a unique technology developed by RIKEN that captures the cap structure at the 5' end of RNA. In this "NET-CAGE" analysis, "NET-RNA," which is currently being synthesized in the nucleus, is extracted and recovered using a unique technique and input for library preparation. (NET-RNA: Negative Elongating-Transcript: newly synthesized RNA) Generally, the lifespan of total RNA is relatively long, ranging from several hours to several days. In contrast, the eRNA (enhancer RNA) from NET-CAGE has an extremely short lifespan, being degraded within just a few minutes. "CAGE" allows us to observe the total RNA present in the cell, while "NET-CAGE" enables us to see the NET-RNA that is currently being synthesized. It has become possible to differentiate between the points we want to observe.